27.03.2020
In vitro testing of Hydroxychloroquine and Azithromycin on SRAS-CoV-2 shows synergistic effect
Therapeutic InfectiologyVirology
Andreani J et al
Pre-prints
Main result
In vitro test for the combination of hydoxychloroquine (H) and/or azythromycin (AZT) at different concentrations.
At low MOI (Multiplicity Of Infection), AZT or H alone had no effect or a low impact on viral production compared to the positive control.
Moderate effect for H at 5 μM. For the combination of AZT and H, inhibition of viral replication at H 5μM + AZT 10μM and H 5μM + AZT 5μM.
At high MOI, neither drug showed any effect. The unique observed effect was with the combination of H at 2μM and AZT at 10μM, leading to total inhibition of viral replication.
Takeaways
Moderate effect of hydroxychloroquine alone on SARS-CoV-2 at low MOI with low concentrations.
Synergistic effect of hydroxychloroquine and azythromycin.
Observed synergy between azithromycin and hydroxychloroquine is consistent with the concentrations achieved in vivo and detected in pulmonary tissues.
Synergistic effect of hydroxychloroquine and azythromycin.
Observed synergy between azithromycin and hydroxychloroquine is consistent with the concentrations achieved in vivo and detected in pulmonary tissues.
Strength of evidence Weak
1) Pre-Prints (no reviewed yet)
2) In vitro
3) No blind test described
4) Descriptive only
Objectives
To study the in vitro synergistic effect of the combination of hydroxychloroquine and azythromycin on SARS-Cov-2.
Method
In vitro study with SARS-CoV-2 strain IHUMI-3, using different concentrations of hydroxychloroquine and azithromycin, alone and in combination, on Vero cells.
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